Multiplex Protein Imaging through PACIFIC: Photoactive Immunofluorescence with Iterative Cleavage.

ABSTRACT: Multiplex protein imaging technologies enable deep phenotyping and provide rich spatial information about biological samples. Existing methods have shown great success but also harbored trade-offs between various pros and cons, underscoring the persisting necessity to expand the imaging toolkits. Here we present PACIFIC: photoactive immunofluorescence with iterative cleavage, a new modality of multiplex protein imaging methods. PACIFIC achieves iterative multiplexing by implementing photocleavable fluorophores for antibody labeling with one-step spin-column purification. PACIFIC requires no specialized instrument, no DNA encoding, or chemical treatments. We demonstrate that PACIFIC can resolve cellular heterogeneity in both formalin-fixed paraffin-embedded (FFPE) samples and fixed cells. T

SUBMITTER: Ji F 

PROVIDER: S-EPMC10288499 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature