A pipeline for sample tagging of whole genome bisulfite sequencing data using genotypes of whole genome sequencing.

ABSTRACT:

Background

In large-scale high-throughput sequencing projects and biobank construction, sample tagging is essential to prevent sample mix-ups. Despite the availability of fingerprint panels for DNA data, little research has been conducted on sample tagging of whole genome bisulfite sequencing (WGBS) data. This study aims to construct a pipeline and identify applicable fingerprint panels to address this problem.

Results

Using autosome-wide A/T polymorphic single nucleotide variants (SNVs) obtained from whole genome sequencing (WGS) and WGBS of individuals from the Third China National Stroke Registry, we designed a fingerprint panel and constructed an optimized pipeline for tagging WGBS data. This pipeline used Bis-SNP to call genotypes from the WGBS data, and optimized genot

SUBMITTER: Xu Z 

PROVIDER: S-EPMC10288677 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature