Differential expression of coagulation pathway-related proteins in diabetic urine exosomes.

ABSTRACT:

Background

Coagulation function monitoring is important for the occurrence and development of diabetes. A total of 16 related proteins are involved in coagulation, but how these proteins change in diabetic urine exosomes is unclear. To explore the expression changes of coagulation-related proteins in urine exosomes and their possible roles in the pathogenesis of diabetes, we performed proteomic analysis and finally applied them to the noninvasive monitoring of diabetes.

Methods

Subject urine samples were collected. LC-MS/MS was used to collect the information on coagulation-related proteins in urine exosomes. ELISA, mass spectrometry and western blotting were used to further verify the differential protein expression in urine exosomes. Correlations with clinical indicators w

SUBMITTER: Liu T 

PROVIDER: S-EPMC10288686 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature