Elevated cell-free mitochondria DNA level of patients with premature ovarian insufficiency.

ABSTRACT:

Background

Premature ovarian insufficiency (POI) patients present with a chronic inflammatory state. Cell-free mitochondria DNA (cf-mtDNA) has been explored as a reliable biomarker for estimating the inflammation-related disorders, however, the cf-mtDNA levels in POI patients have never been measured. Therefore, in the presenting study, we aimed to evaluate the levels of cf-mtDNA in plasma and follicular fluid (FF) of POI patients and to determine a potential role of cf-mtDNA in predicting the disease progress and pregnancy outcomes.

Methods

We collected plasma and FF samples from POI patients, biochemical POI (bPOI) patients and control women. Quantitative real-time PCR was used to measure the ratio of mitochondrial genome to nuclear genome of cf-DNAs extracted from the pla

SUBMITTER: Zhou XY 

PROVIDER: S-EPMC10288775 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature