NEAT1 enhances MPP⁺ -induced pyroptosis in a cell model of Parkinson's disease via targeting miR-5047/YAF2 signaling.

ABSTRACT:

Purpose

Parkinson's disease (PD) is the second most frequent neurodegenerative disease. The aim of our study is to explore the role and the regulatory mechanism of long noncoding RNA (lncRNA) NEAT1 in MPP⁺ -induced pyroptosis in a cell model of PD.

Materials and methods

MPP⁺ -treated SH-SY5Y cells were used as an in vitro model of dopaminergic neurons for PD. Expression levels of miR-5047 and YAF2 mRNA were determined through qRT-PCR. TUNEL staining was carried out to analyze neuronal apoptosis. Luciferase activity assay was accomplished to analyze the combination of miR-5047 with NEAT1 or YAF2 3'-UTR region. Besides, concentrations of IL-1β and IL-18 in supernatant samples were analyzed by using ELISA assay. Expression level of proteins were examined t

SUBMITTER: Shen H 

PROVIDER: S-EPMC10288836 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature