AATF inhibition exerts antiangiogenic effects against human hepatocellular carcinoma.

ABSTRACT:

Background and aims

Angiogenesis is a key factor in the growth and metastasis of hepatic tumors and thus a potential therapeutic target in hepatocellular carcinoma (HCC). In this study, we aim to identify the key role of apoptosis antagonizing transcription factor (AATF) in tumor angiogenesis and its underlying mechanisms in HCC.

Methods

HCC tissues were analyzed for AATF expression by qRT-PCR and immunohistochemistry. Stable clones of control and AATF knockdown (KD) were established in human HCC cells. The effect of AATF inhibition on the angiogenic processes was determined by proliferation, invasion, migration, chick chorioallantoic membrane (CAM) assay, zymography, and immunoblotting techniques.

Results

We identified high levels of AATF in human HCC tissues compare

SUBMITTER: Suresh D 

PROVIDER: S-EPMC10288852 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature