Construction of an alpaca immune antibody library for the selection of nanobodies against Drosophila melanogaster proteins.

ABSTRACT: Introduction: Drosophila melanogaster is a model organism for studying developmental biology and human neural disorders. Nanobodies are the variable domains of the heavy chains of camelid heavy-chain antibodies (VHHs) with high affinity to their antigens and have applications in basic research, similar to traditional antibodies. In addition, nanobodies acting as functionalized antibodies or protein binders have become an additional valuable approach in Drosophila. This study aimed to develop a VHH library against Drosophila proteins and confirm its availability by retrieving some Drosophila protein-specific nanobodies from the library. Methods: An alpaca was first immunized with Drosophila embryo lysate and then its lymphocytes were isolated

SUBMITTER: Qiu J 

PROVIDER: S-EPMC10289234 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature