Hematoxylin Nuclear Stain Reports Oxidative Stress via Near-Infrared Emission.

ABSTRACT: Hematoxylin & eosin (H&E) is the gold standard histological stain used for medical diagnosis and has been used for over a century. Herein, we examined the near-infrared II (NIR-II) fluorescence of this stain. We observed significant NIR-II emission from the hematoxylin component of the H&E stain. We found that the emission intensity, using the common aluminum(III) hematoxylin mordant, could be modulated by the availability of endogenous iron(III), and this emission intensity increased at higher oxidative stress. Our mechanistic investigations found that hematoxylin emission reported the nuclear translocation of the iron via the protein ferritin. In human tumor tissue samples, oxidative stress biomarkers correlated with hematoxylin NIR-II emission intensity. Emission response of the stain w

SUBMITTER: Langenbacher RE 

PROVIDER: S-EPMC10289833 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature