Single-cell ICP-MS for studying the association of inorganic nanoparticles with cell lines derived from aquaculture species.

ABSTRACT: The current research deals with the use of single-cell inductively coupled plasma-mass spectrometry (scICP-MS) for the assessment of titanium dioxide nanoparticle (TiO₂ NP) and silver nanoparticle (Ag NP) associations in cell lines derived from aquaculture species (sea bass, sea bream, and clams). The optimization studies have considered the avoidance of high dissolved background, multi-cell peak coincidence, and possible spectral interferences. Optimum operating conditions were found when using a dwell time of 50 μs for silver and 100 μs for titanium. The assessment of associated TiO₂ NPs by scICP-MS required the use of ammonia as a reaction gas (flow rate at 0.75 mL min^-1) for interference-free titanium determinations (measurements at an m/z ratio of 131

SUBMITTER: Suarez-Oubina C 

PROVIDER: S-EPMC10289938 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature