Imaging conformations of holo- and apo-transferrin on the single-molecule level by low-energy electron holography.

ABSTRACT: Conformational changes play a key role in the biological function of many proteins, thereby sustaining a multitude of processes essential to life. Thus, the imaging of the conformational space of proteins exhibiting such conformational changes is of great interest. Low-energy electron holography (LEEH) in combination with native electrospray ion beam deposition (ES-IBD) has recently been demonstrated to be capable of exploring the conformational space of conformationally highly variable proteins on the single-molecule level. While the previously studied conformations were induced by changes in environment, it is of relevance to assess the performance of this imaging method when applied to protein conformations inherently tied to a function-related conformational change. We show that LEEH i

SUBMITTER: Ochner H 

PROVIDER: S-EPMC10290138 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature