Knockout of PERK protects rat Muller glial cells against OGD-induced endoplasmic reticulum stress-related apoptosis.

ABSTRACT:

Background

The pathological basis for many retinal diseases, retinal ischemia is also one of the most common causes of visual impairment. Numerous ocular diseases have been linked to Endoplasmic reticulum(ER)stress. However, there is still no clear understanding of the relationship between ER stress and Müller glial cells during retinal ischemia and hypoxia. This study examined the effects of ER stress on autophagy and apoptosis-related proteins, as well as the microtubule-related protein tau in rMC-1 cells.

Methods

rMC-1 cells were cultured in vitro. RT-PCR、immunofluorescence and Western blotting revealed the expression levels of associated mRNAs and proteins, and the CCK-8 and flow cytometry assays detected cell apoptosis.

Results

The results showed that under OGD(O

SUBMITTER: Wang X 

PROVIDER: S-EPMC10290337 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature