A PPIX-binding probe facilitates discovery of PPIX-induced cell death modulation by peroxiredoxin.

ABSTRACT: While heme synthesis requires the formation of a potentially lethal intermediate, protoporphyrin IX (PPIX), surprisingly little is known about the mechanism of its toxicity, aside from its phototoxicity. The cellular protein interactions of PPIX might provide insight into modulators of PPIX-induced cell death. Here we report the development of PPB, a biotin-conjugated, PPIX-probe that captures proteins capable of interacting with PPIX. Quantitative proteomics in a diverse panel of mammalian cell lines reveal a high degree of concordance for PPB-interacting proteins identified for each cell line. Most differences are quantitative, despite marked differences in PPIX formation and sensitivity. Pathway and quantitative difference analysis indicate that iron and heme metabolism proteins are pro

SUBMITTER: Lynch J 

PROVIDER: S-EPMC10290680 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature