Structural and dynamical investigation of histone H2B in well-hydrated nucleosome core particles by solid-state NMR.

ABSTRACT: H2A-H2B dimer is a key component of nucleosomes and an important player in chromatin biology. Here, we characterized the structure and dynamics of H2B in precipitated nucleosome core particles (NCPs) with a physiologically relevant concentration using solid-state NMR. Our recent investigation of H3-H4 tetramer determined its unique dynamic properties and the present work provides a deeper understanding of the previously observed dynamic networks in NCP that is potentially functionally significant. Nearly complete ¹³C, ¹⁵N assignments were obtained for H2B R30-A121, which permit extracting unprecedented detailed structural and amino-acid site-specific dynamics. The derived structure of H2B in the well-hydrated NCP sample agrees well with that of X-ray crystals. Dynamic

SUBMITTER: Shi X 

PROVIDER: S-EPMC10290710 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature