Circ_0001387 regulates SKA2 to accelerate breast cancer progression through miR-136-5p.

ABSTRACT:

Background

Growing evidence has revealed the critical regulatory role for circular RNAs (circRNAs) in cancer. This study aimed to explore the function of circ_0001387 in breast cancer (BC).

Methods

Circ_0001387, miR-136-5p, and spindle and kinetochore-associated protein 2 (SKA2) levels were analyzed by quantitative real-time polymerase chain reaction. Clone formation and 5-ethynyl-2'-deoxyuridine assays were used to analyze cell proliferation. Cell apoptosis and cell migration and invasion abilities were analyzed using flow cytometry or transwell assay. Mechanism assay was used to confirm the association between miR-136-5p and circ_0001387 or SKA2. The effect of circ_0001387 on tumor growth in vivo was analyzed by the xenograft mice model.

Results

Circ_0001387 and SKA

SUBMITTER: Xiong Y 

PROVIDER: S-EPMC10290922 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature