Clathrin-mediated endocytosis facilitates the internalization of Magnaporthe oryzae effectors into rice cells.

ABSTRACT: Fungi and oomycetes deliver effectors into living plant cells to suppress defenses and control plant processes needed for infection. Little is known about the mechanism by which these pathogens translocate effector proteins across the plasma membrane into the plant cytoplasm. The blast fungus Magnaporthe oryzae secretes cytoplasmic effectors into a specialized biotrophic interfacial complex (BIC) before translocation. Here, we show that cytoplasmic effectors within BICs are packaged into punctate membranous effector compartments that are occasionally observed in the host cytoplasm. Live cell imaging with fluorescently labeled proteins in rice (Oryza sativa) showed that these effector puncta colocalize with the plant plasma membrane and with CLATHRIN LIGHT CHAIN 1, a component of clathrin-m

SUBMITTER: Oliveira-Garcia E 

PROVIDER: S-EPMC10291035 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature