Coexistence of bla_IMP-4, bla_NDM-1 and bla_OXA-1 in bla_KPC-2-producing Citrobacter freundii of clinical origin in China.

ABSTRACT:

Purpose

To explore the genetic characteristics of the IMP-4, NDM-1, OXA-1, and KPC-2 co-producing multidrug-resistant (MDR) clinical isolate, Citrobacter freundii wang9.

Methods

MALDI-TOF MS was used for species identification. PCR and Sanger sequencing analysis were used to identify resistance genes. In addition to agar dilution, broth microdilution was used for antimicrobial susceptibility testing (AST). We performed whole genome sequencing (WGS) of the strains and analyzed the resulting data for drug resistance genes and plasmids. Phylogenetic trees were constructed with maximum likelihood, plotted using MAGA X, and decorated by iTOL.

Results

Citrobacter freundii carrying bla_KPC-2, bla_IMP-4, bla_OXA-1

SUBMITTER: Qiao J 

PROVIDER: S-EPMC10291173 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature