An improved method for intracellular DNA (iDNA) recovery from terrestrial environments.

ABSTRACT: The simultaneous extraction of intracellular DNA (iDNA) and extracellular DNA (eDNA) can help to separate the living in situ community (represented by iDNA) from background DNA that originated both from past communities and from allochthonous sources. As iDNA and eDNA extraction protocols require separating cells from the sample matrix, their DNA yields are generally lower than direct methods that lyse the cells within the sample matrix. We, therefore, tested different buffers with and without adding a detergent mix (DM) in the extraction protocol to improve the recovery of iDNA from surface and subsurface samples that covered a variety of terrestrial environments. The combination of a highly concentrated sodium phosphate buffer plus DM significantly improved iDNA recovery for almost all t

SUBMITTER: Medina Caro D 

PROVIDER: S-EPMC10291228 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature