A SPECT-based method for dynamic imaging of the glymphatic system in rats.

ABSTRACT: The glymphatic system is a brain-wide waste drainage system that promotes cerebrospinal fluid circulation through the brain to remove waste metabolites. Currently, the most common methods for assessing glymphatic function are ex vivo fluorescence microscopy of brain slices, macroscopic cortical imaging, and MRI. While all these methods have been crucial for expanding our understanding of the glymphatic system, new techniques are required to overcome their specific drawbacks. Here, we evaluate SPECT/CT imaging as a tool to assess glymphatic function in different anesthesia-induced brain states using two radiolabeled tracers, [¹¹¹In]-DTPA and [^99mTc]-NanoScan. Using SPECT, we confirmed the existence of brain state-dependent differences in glymphatic flow and we s

SUBMITTER: Sigurdsson B 

PROVIDER: S-EPMC10291457 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature