Novel method for highly multiplexed gene expression profiling of circulating tumor cells (CTCs) captured from the blood of women with metastatic breast cancer.

ABSTRACT:

Background

Enumeration of circulating tumor cells (CTCs) has proven clinical significance for monitoring patients with metastatic cancers. Multiplexed gene expression profiling of CTCs is a potential tool for assessing disease status and monitoring treatment response. The Parsortix^® technology enables the capture and harvest of CTCs from blood based on cell size and deformability. The HyCEAD^™ (Hybrid Capture Enrichment Amplification and Detection) assay enables simultaneous amplification of short amplicons for up to 100 mRNA targets, and the Ziplex^™ instrument quantifies the amplicons for highly sensitive gene expression profiling down to single cell levels. The aim of the study was to functionally assess this system.

Methods

The HyCEAD/Ziple

SUBMITTER: Farhang Ghahremani M 

PROVIDER: S-EPMC10291750 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature