A novel tyrosine tRNA-derived fragment, tRF^Tyr, induces oncogenesis and lactate accumulation in LSCC by interacting with LDHA.

ABSTRACT:

Background

Transfer (t)RNA-derived small RNA (tsRNA), generated from precursor or mature tRNA, is a new type of small non-coding RNA (sncRNA) that has recently been shown to play a vital role in human cancers. However, its role in laryngeal squamous cell carcinoma (LSCC) remains unclear.

Methods

We elucidated the expression profiles of tsRNAs in four paired LSCC and non-neoplastic tissues by sequencing and verified the sequencing data by quantitative real-time PCR (qRT-PCR) of 60 paired samples. The tyrosine-tRNA derivative tRF^Tyr was identified as a novel oncogene in LSCC for further study. Loss-of-function experiments were performed to evaluate the roles of tRF^Tyr in tumorigenesis of LSCC. Mechanistic experiments including RNA pull-down, parallel reac

SUBMITTER: Zhao R 

PROVIDER: S-EPMC10291753 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature