Neutrophils as key regulators of tumor immunity that restrict immune checkpoint blockade in liver cancer.

ABSTRACT:

Objective

Liver cancer is a deadly malignancy associated with high mortality and morbidity. Less than 20% of patients with advanced liver cancer respond to a single anti-PD-1 treatment. The high heterogeneity of neutrophils in the tumor immune microenvironment in liver cancer may contribute to resistance to immune checkpoint blockade (ICB). However, the underlying mechanism remains largely unknown.

Methods

We established an orthotopic liver cancer model by using transposable elements to integrate the oncogenes Myc and Kras^G12D into the genome in liver cells from conditional Trp53 null/null mice (pTMK/Trp53^-/-). Flow cytometry and immunohistochemistry were used to assess the changes in immune cells in the tumor microenvironment. An ex vi

SUBMITTER: Feng M 

PROVIDER: S-EPMC10291984 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature