AAV11 enables efficient retrograde targeting of projection neurons and enhances astrocyte-directed transduction.

ABSTRACT: Viral tracers that enable efficient retrograde labeling of projection neurons are powerful vehicles for structural and functional dissections of the neural circuit and for the treatment of brain diseases. Currently, some recombinant adeno-associated viruses (rAAVs) based on capsid engineering are widely used for retrograde tracing, but display undesirable brain area selectivity due to inefficient retrograde transduction in certain neural connections. Here we developed an easily editable toolkit to produce high titer AAV11 and demonstrated that it exhibits potent and stringent retrograde labeling of projection neurons in adult male wild-type or Cre transgenic mice. AAV11 can function as a powerful retrograde viral tracer complementary to AAV2-retro in multiple neural connections. In combina

SUBMITTER: Han Z 

PROVIDER: S-EPMC10293207 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature