Contribution of the IGCR1 regulatory element and the 3'Igh CTCF-binding elements to regulation of Igh V(D)J recombination.

ABSTRACT: Immunoglobulin heavy chain variable region exons are assembled in progenitor-B cells, from V_H, D, and J_H gene segments located in separate clusters across the Igh locus. RAG endonuclease initiates V(D)J recombination from a J_H-based recombination center (RC). Cohesin-mediated extrusion of upstream chromatin past RC-bound RAG presents Ds for joining to J_Hs to form a DJ_H-RC. Igh has a provocative number and organization of CTCF-binding elements (CBEs) that can impede loop extrusion. Thus, Igh has two divergently oriented CBEs (CBE1 and CBE2) in the IGCR1 element between the V_H and D/J_H domains, over 100 CBEs across the V_H domain convergent to CBE1, and 10 clustered 3'Igh-CBEs c

SUBMITTER: Liang Z 

PROVIDER: S-EPMC10293834 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature