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Data of germination ability of tetraploid rice lines under multiple stress factors.

ABSTRACT: Rice production is affected by several environmental factors, such as cold, salinity and drought stress. These unfavourable factors could have a serious impact on germination as well as on later growth, causing many types of damage. Recently, polyploid breeding can offer an alternative opportunity to enhance the yield and abiotic stress tolerance in rice breeding. This article describes some germination parameters of 11 different autotetraploid breeding lines and their parental lines under different environmental stresses. Each genotype was grown in a climate chamber under controlled conditions: 13 °C for 4 weeks in the cold test and 30/25 °C for 5 days in control, salinity (150 mM NaCl) and drought (15% PEG 6000) treatments, respectively. The germination process was monitored throughout the experiment. The average data were calculated using three replicates. This dataset contains germination raw data and three calculated germination parameters, such as median germination time (MGT), final germination percentage (FGP), and germination index (GI). These data may provide reliable support to clarify whether the tetraploid lines can exceed the performance of their diploid parental lines under germination phase or not.

SUBMITTER: Szekely A

PROVIDER: S-EPMC10293981 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Data of germination ability of tetraploid rice lines under multiple stress factors.

Székely Árpád Á Szalóki Tímea T Lantos Csaba C Pauk János J Jancsó Mihály M

Data in brief 20230516

Rice production is affected by several environmental factors, such as cold, salinity and drought stress. These unfavourable factors could have a serious impact on germination as well as on later growth, causing many types of damage. Recently, polyploid breeding can offer an alternative opportunity to enhance the yield and abiotic stress tolerance in rice breeding. This article describes some germination parameters of 11 different autotetraploid breeding lines and their parental lines under diffe ...[more]

PMID: 37383734

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Project description:BackgroundRice growth is frequently affected by salinity. When exposed to high salinity, rice seed germination and seedling establishment are significantly inhibited. With the promotion of direct-seeding in Asia, improving rice seed germination under salt stress is crucial for breeding.ResultsIn this study, an indica landrace Wujiaozhan (WJZ) was identified with high germinability under salt stress. A BC1F2 population derived from the crossing WJZ/Nip (japonica, Nipponbare)//Nip, was used to quantitative trait loci (QTL) mapping for the seed germination rate (GR) and germination index (GI) under H2O and 300 mM NaCl conditions. A total of 13 QTLs were identified, i.e. ten QTLs under H2O conditions and nine QTLs under salt conditions. Six QTLs, qGR6.1, qGR8.1, qGR8.2, qGR10.1, qGR10.2 and qGI10.1 were simultaneously identified under two conditions. Under salt conditions, three QTLs, qGR6.2, qGR10.1 and qGR10.2 for GR were identified at different time points during seed germination, which shared the same chromosomal region with qGI6.2, qGI10.1 and qGI10.2 for GI respectively. The qGR6.2 accounted for more than 20% of phenotypic variation under salt stress, as the major effective QTL. Furthermore, qGR6.2 was verified via the BC2F2 population and narrowed to a 65.9-kb region with eleven candidate genes predicted. Based on the microarray database, five candidate genes were found with high transcript abundances at the seed germination stage, of which LOC_Os06g10650 and LOC_Os06g10710 were differentially expressed after seed imbibition. RT-qPCR results showed the expression of LOC_Os06g10650 was significantly up-regulated in two parents with higher levels in WJZ than Nip during seed germination under salt conditions. Taken together, it suggests that LOC_Os06g10650, encoding tyrosine phosphatase family protein, might be the causal candidate gene for qGR6.2.ConclusionsIn this study, we identified 13 QTLs from a landrace WJZ that confer seed germination traits under H2O and salt conditions. A major salt-tolerance-specific QTL qGR6.2 was fine mapped to a 65.9-kb region. Our results provide information on the genetic basis of improving rice seed germination under salt stress by marker-assisted selection (MAS).

Dataset Information

Data of germination ability of tetraploid rice lines under multiple stress factors.

Publications

Data of germination ability of tetraploid rice lines under multiple stress factors.

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