Pharmacologic inhibition of IL11/STAT3 signaling increases MHC-I expression and T cell infiltration.

ABSTRACT:

Background

Recent studies have discovered an emerging role of IL11 in various colitis-associated cancers, suggesting that IL11 mainly promotes tumor cell survival and proliferation in regulating tumorigenesis. Herein we aimed to reveal a novel function of IL-11 through STAT3 signaling in regulating tumor immune evasion.

Methods

AOM/DSS model in Il11^-/- and Apc^min/+/Il11^-/- mice were used to detect tumor growth and CD8⁺ T infiltration. STAT1/3 phosphorylation and MHC-I, CXCL9, H2-K1 and H2-D1 expression were detected in MC38 cells and intestine organoids treated with/without recombinant IL11 to explore effect of IL11/STAT3 signaling, with IL11 mutein used to competitively inhibit IL11 and rescue inhibited STAT1 activation. Correlati

SUBMITTER: Xiong W 

PROVIDER: S-EPMC10294373 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature