Enpp1 deficiency caused chondrocyte apoptosis by inhibiting AMPK signaling pathway.

ABSTRACT:

Objective and background

The deficiency of ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1) causes the phenotype similar to knee osteoarthritis (OA). However, the molecular mechanism is poorly understood.

Method

The global deletion of Enpp1 (Enpp1^-/-) mice was created to analyze the role of Enpp1 in the progress of knee OA. The apoptosis, proliferation and chondrogenic differentiation ability of chondrocytes from wild-type (WT) and Enpp1^-/- joints were compared. According to the results of high-throughput quantitative molecular measurements, the proteins of chondrocytes from WT and Enpp1^-/- mice were used to explore the mechanism of Enpp1 deficiency-associated knee OA.

Result

In Enpp1^-/- knee joints, we found signi

SUBMITTER: Gao Z 

PROVIDER: S-EPMC10294376 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature