Therapeutic efficacy of sorafenib and plant-derived phytochemicals in human colorectal cancer cells.

ABSTRACT:

Background

The present study aimed to investigate the sequence-dependent anticancer effects of combined treatment with sorafenib (Sora), a Food and Drug Administration-approved multikinase inhibitor drug, and plant-derived phytochemicals (PPCs) on human colorectal cancer (CRC) cell growth, and proteins associated with the control of cell cycle and apoptosis.

Methods

The cytotoxic effects of 14 PPCs on CRL1554 fibroblast cells were determined using an MTT assay. Moreover, the cytotoxicity of Sora, PPCs, and a combination of both on CRC cells were also investigated. Cell cycle analysis was performed using flow cytometry, and cell apoptosis was investigated using DNA fragmentation, Annexin V/propidium iodide double staining, and mitochondrial membrane potential analyses. The ce

SUBMITTER: Bahman A 

PROVIDER: S-EPMC10294390 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature