A Sporulation-Independent Way of Life for Bacillus thuringiensis in the Late Stages of an Infection.

ABSTRACT: The formation of endospores has been considered the unique survival and transmission mode of sporulating Firmicutes due to the exceptional resistance and persistence of this bacterial form. However, nonsporulated bacteria (Spo^-) were reported at the early stages following the death of a host infected with Bacillus thuringiensis, an entomopathogenic sporulating bacterium. Here, we investigated the characteristics of the bacterial population in the late stages of an infection in the B. thuringiensis/Galleria mellonella infection model. Using fluorescent reporters and molecular markers coupled to flow cytometry, we demonstrated that the Spo^- cells persist and constitute about half of the population 2 weeks post-infection (p.i.). Protein synthesis and growth recove

SUBMITTER: Toukabri H 

PROVIDER: S-EPMC10294645 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature