A method for the efficient evaluation of substrate-based cholinesterase imaging probes for Alzheimer's disease.

ABSTRACT: Cholinesterase (ChE) enzymes have been identified as diagnostic markers for Alzheimer disease (AD). Substrate-based probes have been synthesised to detect ChEs but they have not detected changes in ChE distribution associated with AD pathology. Probes are typically screened using spectrophotometric methods with pure enzyme for specificity and kinetics. However, the biochemical properties of ChEs associated with AD pathology are altered. The present work was undertaken to determine whether the Karnovsky-Roots (KR) histochemical method could be used to evaluate probes at the site of pathology. Thirty thioesters and esters were synthesised and evaluated using enzyme kinetic and KR methods. Spectrophotometric methods demonstrated all thioesters were ChE substrates, yet only a few provided stai

SUBMITTER: Darvesh S 

PROVIDER: S-EPMC10294744 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature