Injectable biomaterial induces regeneration of the intervertebral disc in a caprine loaded disc culture model.

ABSTRACT: Back pain is the leading cause of disability with half of cases attributed to intervertebral disc (IVD) degeneration, yet currently no therapies target this cause. We previously reported an ex vivo caprine loaded disc culture system (LDCS) that accurately represents the cellular phenotype and biomechanical environment of human IVD degeneration. Here, the efficacy of an injectable hydrogel system (LAPONITE® crosslinked pNIPAM-co-DMAc, (NPgel)) to halt or reverse the catabolic processes of IVD degeneration was investigated within the LDCS. Following enzymatic induction of degeneration using 1 mg mL^-1 collagenase and 2 U mL^-1 chondroitinase ABC within the LDCS for 7 days, IVDs were injected with NPgel alone or with encapsulated human bone marrow progenitor

SUBMITTER: Snuggs JW 

PROVIDER: S-EPMC10294806 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature