Prediction of Metabolic Flux Distribution by Flux Sampling: As a Case Study, Acetate Production from Glucose in Escherichia coli.

ABSTRACT: Omics data was acquired, and the development and research of metabolic simulation and analysis methods using them were also actively carried out. However, it was a laborious task to acquire such data each time the medium composition, culture conditions, and target organism changed. Therefore, in this study, we aimed to extract and estimate important variables and necessary numbers for predicting metabolic flux distribution as the state of cell metabolism by flux sampling using a genome-scale metabolic model (GSM) and its analysis. Acetic acid production from glucose in Escherichia coli with GSM iJO1366 was used as a case study. Flux sampling obtained by OptGP using 1000 pattern constraints on substrate, product, and growth fluxes produced a wider sample than the default case. The an

SUBMITTER: Kuriya Y 

PROVIDER: S-EPMC10295088 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature