Exogenous 8-Hydroxydeoxyguanosine Attenuates PM_2.5-Induced Inflammation in Human Bronchial Epithelial Cells by Decreasing NLRP3 Inflammasome Activation.

ABSTRACT: Particulate matter 2.5 (PM_2.5) induces lung injury by increasing the generation of reactive oxygen species (ROS) and inflammation. ROS aggravates NLRP3 inflammasome activation, which activates caspase-1, IL-1β, and IL-18 and induces pyroptosis; these factors propagate inflammation. In contrast, treatment with exogenous 8-hydroxydeoxyguanosine (8-OHdG) decreases RAC1 activity and eventually decreases dinucleotide phosphate oxidase (NOX) and ROS generation. To establish modalities that would mitigate PM_2.5-induced lung injury, we evaluated whether 8-OHdG decreased PM_2.5-induced ROS generation and NLRP3 inflammasome activation in BEAS-2B cells. CCK-8 and lactate dehydrogenase assays were used to determine the treatment concentration. Fluorescence intensity, We

SUBMITTER: Bang J 

PROVIDER: S-EPMC10295443 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature