Functional Analysis of GSTK1 in Peroxisomal Redox Homeostasis in HEK-293 Cells.

ABSTRACT: Peroxisomes serve as important centers for cellular redox metabolism and communication. However, fundamental gaps remain in our understanding of how the peroxisomal redox equilibrium is maintained. In particular, very little is known about the function of the nonenzymatic antioxidant glutathione in the peroxisome interior and how the glutathione antioxidant system balances with peroxisomal protein thiols. So far, only one human peroxisomal glutathione-consuming enzyme has been identified: glutathione S-transferase 1 kappa (GSTK1). To study the role of this enzyme in peroxisomal glutathione regulation and function, a GSTK1-deficient HEK-293 cell line was generated and fluorescent redox sensors were used to monitor the intraperoxisomal GSSG/GSH and NAD⁺/NADH redox couples and NADP

SUBMITTER: Costa CF 

PROVIDER: S-EPMC10295636 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature