Uncovering the Important Genetic Factors for Growth during Cefotaxime-Gentamicin Combination Treatment in bla_CTX-M-1 Encoding Escherichia coli.

ABSTRACT: Due to the rapid spread of CTX-M type ESBLs, the rate of resistance to third-generation cephalosporin has increased among Gram-negative bacteria, especially in Escherichia coli, and there is a need to find ways to re-sensitize ESBL E. coli to cephalosporin treatment. A previous study showed that genes involved in protein synthesis were significantly up-regulated in the presence of subinhibitory concentration of cefotaxime (CTX) in a CTX-M-1-producing E. coli. In this study, the interaction between CTX and gentamicin (GEN), targeting protein synthesis, was evaluated in MG1655/pTF2, and the MIC of CTX was strongly reduced (128-fold) in the presence of this combnation therapy. Since the underlying mechanism behind this synergy is not known, we constructed a saturated tran

SUBMITTER: Alobaidallah MSA 

PROVIDER: S-EPMC10295648 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature