RNA Sequencing of Pooled Samples Effectively Identifies Differentially Expressed Genes.

ABSTRACT: Analysis of gene expression changes across the genome provides a powerful, unbiased tool for gaining insight into molecular mechanisms. We have effectively used RNA sequencing to identify differentially expressed genes in long-lived genetic mutants in C. elegans to advance our understanding of the genetic pathways that control longevity. Although RNA sequencing costs have come down, cost remains a barrier to examining multiple strains and time points with a sufficient number of biological replicates. To circumvent this, we have examined the efficacy of identifying differentially expressed genes by sequencing a pooled RNA sample from long-lived isp-1 mitochondrial mutant worms. We found that sequencing a pooled RNA sample could effectively identify genes that were found to be

SUBMITTER: Ko B 

PROVIDER: S-EPMC10295764 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature