Multi-Probe Nano-Genomic Biosensor to Detect S. aureus from Magnetically-Extracted Food Samples.

ABSTRACT: One of the most prevalent causes of foodborne illnesses worldwide is staphylococcal food poisoning. This study aimed to provide a robust method to extract the bacteria Staphylococcus aureus from food samples using glycan-coated magnetic nanoparticles (MNPs). Then, a cost-effective multi-probe genomic biosensor was designed to detect the nuc gene of S. aureus rapidly in different food matrices. This biosensor utilized gold nanoparticles and two DNA oligonucleotide probes combined to produce a plasmonic/colorimetric response to inform users if the sample was positive for S. aureus. In addition, the specificity and sensitivity of the biosensor were determined. For the specificity trials, the S. aureus biosensor was compared with the extracted DNA of Escherichia

SUBMITTER: Boodoo C 

PROVIDER: S-EPMC10295863 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature