Label-Free Electrochemical Aptasensor Based on the Vertically-Aligned Mesoporous Silica Films for Determination of Aflatoxin B1.

ABSTRACT: Herein we report a highly specific electrochemical aptasenseor for AFB1 determination based on AFB1-controlled diffusion of redox probe (Ru(NH₃)₆³⁺) through nanochannels of AFB1-specific aptamer functionalized VMSF. A high density of silanol groups on the inner surface confers VMSF with cationic permselectivity, enabling electrostatic preconcentration of Ru(NH₃)₆³⁺ and producing amplified electrochemical signals. Upon the addition of AFB1, the specific interaction between the aptamer and AFB1 occurs and generates steric hindrance effect on the access of Ru(NH₃)₆³⁺, finally resulting in the reduced electrochemical responses and allowing the quantitative determination of AFB1. The proposed elec

SUBMITTER: Zhang T 

PROVIDER: S-EPMC10296088 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature