Dysfunctions of Neutrophils in the Peripheral Blood of Children with Cystic Fibrosis.

ABSTRACT: Dysfunction of neutrophils in patients with cystic fibrosis (CF) is best characterized in bronchoalveolar lavage (BAL), whereas peripheral blood neutrophils are less examined, and the results are contradictory, especially in younger populations. Therefore, this work aimed to study functional and phenotypic changes in circulating neutrophils in children with CF. The study included 19 CF children (5-17 years) and 14 corresponding age-matched healthy children. Isolated neutrophils were cultured either alone or with different stimuli. Several functions were studied: apoptosis, NET-osis, phagocytosis, and production of reactive oxygen species (ROS), neutrophil elastase (NE), and 11 cytokines. In addition, the expression of 20 molecules involved in different functions of neutrophils was evaluate

SUBMITTER: Bakalovic G 

PROVIDER: S-EPMC10296301 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature