Synthesis of Fe-Doped Peroxidase Mimetic Nanozymes from Natural Hemoglobin for Colorimetric Biosensing and In Vitro Anticancer Effects.

ABSTRACT: Despite their efficiency and specificity, the instability of natural enzymes in harsh conditions has inspired researchers to replace them with nanomaterials. In the present study, extracted hemoglobin from blood biowastes was hydrothermally converted to catalytically active carbon nanoparticles (BDNPs). Their application as nanozymes for the colorimetric biosensing of H₂O₂ and glucose and selective cancer cell-killing ability was demonstrated. Particles that were prepared at 100 °C (BDNP-100) showed the highest peroxidase mimetic activity, with Michaelis-Menten constants (K_m) of 11.8 mM and 0.121 mM and maximum reaction rates (V_max) of 8.56 × 10^-8 mol L^-1 s^-1 and 0.538 × 10^-8 mol L^-1 s^-1

SUBMITTER: Mohammadpour Z 

PROVIDER: S-EPMC10296309 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature