YGPS-P: A Yeast-Based Peptidome Screen for Studying Quality Control-Associated Proteolysis.

ABSTRACT: Quality control-associated proteolysis (QCAP) is a fundamental mechanism that maintains cellular homeostasis by eliminating improperly folded proteins. In QCAP, the exposure of normally hidden cis-acting protein sequences, termed degrons, triggers misfolded protein ubiquitination, resulting in their elimination by the proteasome. To identify the landscape of QCAP degrons and learn about their unique features we have developed an unbiased screening method in yeast, termed yGPS-P, which facilitates the determination of thousands of proteome-derived sequences that enhance proteolysis. Here we describe the fundamental features of the yGPS-P method and provide a detailed protocol for its use as a tool for degron search. This includes the cloning of a synthetic peptidome library in a fluo

SUBMITTER: Mashahreh B 

PROVIDER: S-EPMC10296357 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature