BPA Endocrine Disruptor Detection at the Cutting Edge: FPIA and ELISA Immunoassays.

ABSTRACT: BPA is a chemical commonly used in the production of polymer-based materials that can have detrimental effects on the thyroid gland and impact human reproductive health. Various expensive methods, such as liquid and gas chromatography, have been suggested for detecting BPA. The fluorescence polarization immunoassay (FPIA) is an inexpensive and efficient homogeneous mix-and-read method that allows for high-throughput screening. FPIA offers high specificity and sensitivity and can be carried out in a single phase within a timeframe of 20-30 min. In this study, new tracer molecules were designed that linked the fluorescein fluorophore with and without a spacer to the bisphenol A moiety. To assess the influence of the C6 spacer on the sensitivity of an assay based on the respective antibody, h

SUBMITTER: Raysyan A 

PROVIDER: S-EPMC10296492 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature