Development of an Automated Online Flow Cytometry Method to Quantify Cell Density and Fingerprint Bacterial Communities.

ABSTRACT: Cell density is an important factor in all microbiome research, where interactions are of interest. It is also the most important parameter for the operation and control of most biotechnological processes. In the past, cell density determination was often performed offline and manually, resulting in a delay between sampling and immediate data processing, preventing quick action. While there are now some online methods for rapid and automated cell density determination, they are unable to distinguish between the different cell types in bacterial communities. To address this gap, an online automated flow cytometry procedure is proposed for real-time high-resolution analysis of bacterial communities. On the one hand, it allows for the online automated calculation of cell concentrations and, o

SUBMITTER: Lopez-Galvez J 

PROVIDER: S-EPMC10296507 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature