A Truncated 14-Amino-Acid Myelin Protein-Zero-Targeting Peptide for Fluorescence-Guided Nerve-Preserving Surgery.

ABSTRACT:

Background

The occurrence of accidental nerve damage during surgery and the increasing application of image guidance during head-and-neck surgery have highlighted the need for molecular targeted nerve-sparing interventions. The implementation of such interventions relies on the availability of nerve-specific tracers. In this paper, we describe the development of a truncated peptide that has an optimized affinity for protein zero (P0), the most abundant protein in myelin.

Methods and materials

Further C- and N-terminal truncation was performed on the lead peptide Cy5-P0_101-125. The resulting nine Cy5-labelled peptides were characterized based on their photophysical properties, P0 affinity, and in vitro staining. These characterizations were combined with evaluation

SUBMITTER: Berehova N 

PROVIDER: S-EPMC10296720 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature