Detection of c.375A>G, c.385A>T, c.571C>T, and se^del2 of FUT2 via Real-Time PCR in a Single Tube.

ABSTRACT: α(1,2)fucosyltransferase (Se enzyme) encoded by FUT2 is involved in the secretor status of ABH(O) blood group antigens. The se^del2 allele is one of the non-functional FUT2 (se) alleles in which 9.3 kb, containing the entire coding region of FUT2, is deleted by Alu-mediated nonhomologous recombination. In addition to this allele, three SNPs of FUT2, c.375A>G, c.385A>T, and c.571C>T, appear to be prevalent in certain Oceanian populations such as Polynesians. Recently, we developed an endpoint genotyping assay to determine se^del2 zygosity, using a FAM-labeled probe for detection of the se^del2 allele and a VIC-labeled probe for the detection of FUT2. In this study, instead of the VIC probe, a

SUBMITTER: Soejima M 

PROVIDER: S-EPMC10297093 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature