Differences in the neural basis and transcriptomic patterns in acute and persistent pain-related anxiety-like behaviors.

ABSTRACT:

Background

Both acute and persistent pain is associated with anxiety in clinical observations, but whether the underlying neural mechanisms differ is poorly understood.

Methods

We used formalin or complete Freund's adjuvant (CFA) to induce acute or persistent pain. Behavioral performance was assessed by the paw withdrawal threshold (PWT), open field (OF), and elevated plus maze (EPM) tests. C-Fos staining was used to identify the activated brain regions. Chemogenetic inhibition was further performed to examine the necessity of brain regions in behaviors. RNA sequencing (RNA-seq) was used to identify the transcriptomic changes.

Results

Both acute and persistent pain could lead to anxiety-like behavior in mice. The c-Fos expression indicates that the bed nucleus of the

SUBMITTER: Fang S 

PROVIDER: S-EPMC10297165 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature