Assembly of Synaptic Protein-DNA Complexes: Critical Role of Non-Specific Interactions.

ABSTRACT: The synaptic protein-DNA complexes, formed by specialized proteins that bridge two or more distant sites on DNA, are critically involved in various genetic processes. However, the molecular mechanism by which the protein searches for these sites and how it brings them together is not well understood. Our previous studies directly visualized search pathways used by SfiI, and we identified two pathways, DNA threading and site-bound transfer pathways, specific to the site-search process for synaptic DNA-protein systems. To investigate the molecular mechanism behind these site-search pathways, we assembled complexes of SfiI with various DNA substrates corresponding to different transient states and measured their stability using a single-molecule fluorescence approach. These assemblies corresp

SUBMITTER: Vemulapalli S 

PROVIDER: S-EPMC10298106 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature