Features of DNA-Montmorillonite Binding Visualized by Atomic Force Microscopy.

ABSTRACT: In the present work, complexes of DNA with nano-clay montmorillonite (Mt) were investigated by means of atomic force microscopy (AFM) under various conditions. In contrast to the integral methods of analysis of the sorption of DNA on clay, AFM allowed us to study this process at the molecular level in detail. DNA molecules in the deionized water were shown to form a 2D fiber network weakly bound to both Mt and mica. The binding sites are mostly along Mt edges. The addition of Mg²⁺ cations led to the separation of DNA fibers into separate molecules, which bound mainly to the edge joints of the Mt particles according to our reactivity estimations. After the incubation of DNA with Mg²⁺, the DNA fibers were capable of wrapping around the Mt particles and were weakly bound

SUBMITTER: Kraevsky SV 

PROVIDER: S-EPMC10298354 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature