Expression of transglutaminase 2 in human gut epithelial cells: Implications for coeliac disease.

ABSTRACT:

Background

Formation of complexes between transglutaminase 2 (TG2) and gluten can mechanistically explain why TG2 serves both as B-cell autoantigen and as an enzyme that creates deamidated gluten epitopes in coeliac disease (CeD). A model has been proposed where TG2 released from shed epithelial cells encounters high concentrations of dietary gluten peptides to form these TG2:gluten complexes. In this work we have characterised TG2 protein expression in gut epithelial cells in humans.

Methods

Western blot analysis, immunofluorescence staining and mass spectrometry in combination with laser capture microdissection to gain spatial resolution were used to characterise TG2 expression in the epithelial cell layer of healthy and coeliac disease affected duodenum.

Findings

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SUBMITTER: Amundsen SF 

PROVIDER: S-EPMC10298751 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature