Heterologous expression of genes from a cyanobacterial endosymbiont highlights substrate exchanges with its diatom host.

ABSTRACT: A few genera of diatoms are widespread and thrive in low-nutrient waters of the open ocean due to their close association with N₂-fixing, filamentous heterocyst-forming cyanobacteria. In one of these symbioses, the symbiont, Richelia euintracellularis, has penetrated the cell envelope of the host, Hemiaulus hauckii, and lives inside the host cytoplasm. How the partners interact, including how the symbiont sustains high rates of N₂ fixation, is unstudied. Since R. euintracellularis has evaded isolation, heterologous expression of genes in model laboratory organisms was performed to identify the function of proteins from the endosymbiont. Gene complementation of a cyanobacterial invertase mutant and expression of the protein in Escherichia coli</

SUBMITTER: Nieves-Morion M 

PROVIDER: S-EPMC10299089 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature