Three-dimensional cellular visualization in mouse apical periodontitis using combined whole-mount staining and optical tissue clearing.

ABSTRACT: Apical periodontitis is an inflammatory disease involving lesions located within the jawbone. Histological evaluations generally require decalcification and sectioning, which has limited our understanding of the three-dimensional (3D) organization and spatial distribution of different immune cell types in these lesions. A recently developed technique combining tissue clearing and whole-mount immunofluorescent labeling allows us to acquire such information from the deep tissue without sectioning. However, whole-mount immunofluorescent labeling in the jawbone requires further development. Here we provide a straightforward and efficient protocol to achieve 3D immunofluorescent imaging of murine periapical lesions.

SUBMITTER: Tazawa K 

PROVIDER: S-EPMC10299740 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature